Our research interests include but not limited to:
Protein physical biology, including liquid-liquid phase separation
Protein-protein interaction
Oncoprotein signaling network
Signaling reporter design
Animal development, including embryogenesis
Molecular mechanisms of various diseases including cancer and neurodegeneration
"If you're not having fun, you are not learning. There's a pleasure in finding things out." - Richard Feynman
1) Having fun is an essential part of our research life.
Our students and postdoc trainees and the PI always find unexpected fun in their biological adventures because we are an interdisciplinary lab, focusing on visualizing inner life of living cells and animals.
The cells' inner life is like a glowing beautiful world of Avatar, after we label many proteins in the cell with multicolor fluorescent reporters.
2) Our research interests span the fields of Physical Biology, Chemical Biology, Structural Biology, Protein Engineering, Cell & Molecular Biology, Developmental Biology, and Drug Discovery.
We apply Physics, Chemistry and Engineering to design biological tools for visualizing and manipulating dynamic cell signaling in vivo in order to understand animal development and homeostasis, because dynamical features of signaling are the essence of living organisms.
Effector proteins such as proteases and kinases regulate almost every major signaling pathways, and protein-protein interactions (PPIs) define specificity of signal transduction from extracellular cues to the appropriate intracellular effector proteins.
3) We have developed new classes of fluorescent reporters of proteases, kinases, and PPIs.
These reporters achieve large dynamic range, high brightness and fast kinetics, in addition being genetically encoded requiring no exogenous cofactor for non-invasive imaging.
Our reporters enable robust and rapid imaging of dynamic signaling in living cells and animals with unexpected fun, identifying small molecules that inhibit dysregulated signaling in disease such as cancer.
4) Other biological tools we are developing include:
Chemogenetic tools for manipulating protein interaction and liquid-liquid phase separation.
A genetically encoded photosensitizer for activating caspase and apoptosis signaling and ablating single cells in live animals; singlet oxygen-mediated proximity labeling technologies for identifying weak and transient PPIs by mass spectrometry
5) We are applying these novel technologies to explore exciting biology and therapeutics.
We have shared these tools by depositing them to not-for-profit organizations, e.g. Addgene, Bloomington Stock Center.
Positions are available for highly motivated, independently thinking individuals.
Laboratory of Xiaokun Shu, PhD | University of California, San Fancisco | Advancing Health Worldwide
